Intermediate

Part:BBa_K855000:Design

Designed by: Akansha Mahavir Shah, Ma Tsz Shan, Nelson Chu   Group: iGEM12_HKU_HongKong   (2012-09-20)


pvdQ-B0015


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 1489
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 1489
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1196
    Illegal XhoI site found at 1594
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 1489
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 1489
    Illegal NgoMIV site found at 31
    Illegal NgoMIV site found at 770
    Illegal NgoMIV site found at 1172
    Illegal NgoMIV site found at 1329
    Illegal NgoMIV site found at 1556
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 1926


Design Notes

The pvdQ gene consists of a EcoRI-like site. This means that although the exact EcoRI sequence is not present , the EcoRI restriction enzyme is still capable of cutting pvdQ once. Thereby, a short digestion time is needed to lessen this star activity.


Source

Pseudomonas aeruginosa PAO1

References